Cell-Free Protein Expression under Macromolecular Crowding Conditions
نویسندگان
چکیده
منابع مشابه
Cell-Free Protein Expression under Macromolecular Crowding Conditions
BACKGROUND Cell-free protein expression (CFPE) comprised of in vitro transcription and translation is currently manipulated in relatively dilute solutions, in which the macromolecular crowding effects present in living cells are largely ignored. This may not only affect the efficiency of protein synthesis in vitro, but also limit our understanding of the functions and interactions of biomolecul...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2011
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0028707